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| Features | Bio-Dot | Bio-Dot SF |
| Format | Dot blot | Slot blot |
| Samples | 96-well, 8 x 12 format | 48-well, 6 x 8 format |
| Sample volume | 50–600 µl | 50–500 µl |
| Well size | 3 mm diameter | 7 x 0.75 mm |
| Quantitation with densitometer | Yes, but Bio-Dot SF Unit Recommended | Yes |
| Overnight incubations | Yes | No |
| Membrane size (W x L) | 12 x 9 cm | 12 x 9 cm |
| Dimensions (W x H x D) | 9 x 6.5 x 12" | 9.5 x 7 x 12" |
| Weight | 1.1 kg (2.5 lb) | 1.2 kg (2.6 lb) |
